Correct evaluation of entrepreneurial opportunities could enhance the likelihood of success and neglecting of this process could cause business failure. This study seeks to identify what are the significant factors in the evaluation of business opportunities in organic food industry and what is the degree of the importance of each factor. The research statistical populations were entrepreneurs of organic food industry in Golestan, Isfahan, Markazi, Gilan and Khorasan in 2013, that 16 of them selected based on Krejcie and Morgan tables. The most important tool of data collection was the standard questionnaire, whose validity was confirmed by specialists and its reliability was computed using Inconsistency ratio (0.08), which shows its reliability. The results indicated that evaluation of the business opportunities in organic foods sector include five factors and thirty-two sub factors. The most important factor is market and the organic segment, characteristics of the entrepreneur, financial, investment and technology, are prioritized respectively. The proposed framework in this paper which indicates the main criteria on evaluating entrepreneurial opportunities is a good reference for future survey researches.