Business enterprises are places for growth and progress of entrepreneurs. Businesses and entrepreneurship play key role in economic development of various communities. The purpose of this research was to identify difficulties of agricultural businesses in animal sub-sector of Ilam Township. This research was applied and a descriptive-survey method was used. Population of this study was 952 managers of enterprises which by using of proportionate stratified random sampling method and use of Cochran formula 120 enterprises were selected. Instrument of data gathering was questionnaire that its validity confirmed by expert panel and for reliability of questionnaire, cronbach alpha coefficient was used that its value was equal to 0.93 that is acceptable. Descriptive statistics such as mean, frequency and coefficient of variance were used for data analysis. Difficulties of businesses were categorized by factor analysis. Results showed that difficulties of infrastructure-public(%23/40), economic(%15/15), marketing(%13/25), management(%10/03), educational(%4/24) and sociocultural(%3/10) have identified as the main difficulties of agricultural businesses that these factors explain %69/178 of total variance.